|Phillips, Ronald - UNIVERSITY OF MINNESOTA|
Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: October 31, 1999
Publication Date: N/A
Technical Abstract: Embryo rescue procedures allow the recovery of partially fertile plants from the pollination of oat plants with corn pollen. These plants are oat haploids either with or without corn chromosomes. Some seed is set when the plants are haploid with 21 oat chromosomes or haploid with one or two corn chromosomes. Meiotic restitution produces viable gametes that generate euploids and aneuploids with only oat chromosomes or oat-corn chromosome addition lines most often with 42 oat chromosomes plus a pair of corn chromosomes (disomic addition lines). The phenotypes of the addition lines with one or two corn chromosomes may be near-normal or reflect some modification depending on the chromosome. An efficient system for mapping corn genes involving radiation hybrid lines is being developed via the irradiation of oat-corn chromosome addition lines. The objectives include generating approximately 100 radiation hybrid lines per chromosome and using genetic markers to define the breakpoints. At this point, we have analyzed 37 radiation hybrid lines for chromosome 9 with 56 markers; this analysis indicates that an unknown sequence hybridized to DNAs of these lines would allow the mapping of that sequence to one of 42 bins for chromosome 9. The chromosome addition and radiation hybrid lines are being screened for specialized traits that might be introgressed from corn into oat.