|Post, Karen - NC DEPT.AG.,RALEIGH, NC.|
Submitted to: Biochemistry and Molecular Biology Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: November 13, 1999
Publication Date: N/A
Technical Abstract: Haemophilus parasuis is the causative agent of Glasser's disease, manifested as pleuritis, polyserositis and meningitis. H. parasuis is considered a re-emerging pathogen of swine and its incidence is related to the introduction of new management practices, such as segregated early weaning (SEW). Even though H. parasuis is a commensal of the normal flora of the upper respiratory tract, outbreaks of H. parasuis infection are believed to occur as the result of stress, and/or co-infection with other bacterial or viral pathogens. Very little information is available on H. parasuis proteins. The objective of this study is to identify some of the major immunoreactive proteins for the development of improved vaccines and/or identification of diagnostic reagents for the development of a "quick" on-site diagnostic test for H. parasuis. Using Western blotting, we have identified a major immunoreactive membrane-anchored protein of H. parasuis serovars 4 and 5 with a molecular weight of 46 kDa. N-terminal amino acid sequencing information from two internal fragments obtained after Lys-C digestion followed by C-18 reversed-phase HPLC identified the protein in the H. influenzae database. The 46 kDa protein may serve as one of the diagnostic antigens of H. parasuis.