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United States Department of Agriculture

Agricultural Research Service

Title: Transmission and Expression of An Antifungal Protein and the Green Fluorescent Protein Gene in Progeny of Transgenic Oat Plants Generated Using a Visual Selection System

Authors
item Kaeppler, Heidi - UNIV OF WISCONSIN-MADISON
item Menon, Geeta - UNIV OF WISCONSIN-MADISON
item Skadsen, Ronald

Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: October 1, 1998
Publication Date: N/A

Technical Abstract: A new selection system based solely on visual selection for cells expressing the green fluorescent protein, gfp, was used to obtain fertile, transgenic oat plants. Transgenic regenerant (T0) and progeny plants (T1) from 6 unique transformation events were analyzed for transmission and expression of the gfp gene. Additionally, 3 of the 6 transgenic lines were cotransformed with a gene encoding the antifungal protein, permatin. Expression of gfp was observed in all T0 plants. Expression of gfp in the T1 segregated in a 3:1 ratio. Molecular analysis confirmed integration of both the gfp and permatin genes in T0 and T1 plants. T0 and regenerated control (R0) plants were shorter, and in general, less fertile than (S1) plants grown from seeds of noncultured, control plants. Fertility of gfp-expressing T1 plants and nonexpressing R0 plants was identical. Growth and morphology of T1 and R1 plants was similar to that of S1 seedlings. Results of further analysis of both gfp and permatin expression will be reported.

Last Modified: 4/18/2014
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