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United States Department of Agriculture

Agricultural Research Service

Title: DNA Fingerprinting of Mycobacterium Bovis from Animals in Michigan

Authors
item Whipple, Diana
item Janagin, J - USDA, APHIS
item Payeur, J - USDA, APHIS

Submitted to: International Workshop on Tuberculosis in Animals
Publication Type: Abstract Only
Publication Acceptance Date: September 15, 1999
Publication Date: N/A

Technical Abstract: Restriction fragment length polymorphism (RFLP) analysis and spoligotyping were used to study the molecular epidemiology of Mycobacterium bovis isolates from over 145 wild white-tailed deer (WTD), three captive WTD, five coyotes, two raccoons and one bear harvested from a five county region of northeastern Michigan (MI). Isolates from three cattle herds in MI and one heifer from Wisconsin (WI) also were analyzed. DNA was extracted from each isolate and digested with restriction endonucleases PvuII and AluI. The following DNA probes were used: 1) an IS6110 123-bp sequence; 2) an IS6110 445-bp sequence; 3) the direct repeat sequence; and 4) the polymorphic GC-rich repetitive sequence. RFLP types were designated for each isolate by combining results of the 4 different probes. Spoligotyping was done as previously described. Approximately 95% of the isolates from the WTD had a single copy of IS6110. The RFLP patterns for about 81% of the wild deer isolates were identical to the patterns for isolates from the captive deer, coyotes, raccoons, bear, MI cattle, and WI heifer. The remaining deer isolates had minor differences in RFLP patterns. Results of spoligotyping were identical for all except two deer isolates, which differed by the absence of one spacer sequence. Results of this study indicate that animals with tuberculosis in northeast MI are infected with a common strain of M. bovis.

Last Modified: 7/23/2014
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