Submitted to: United States Japan Natural Resources Protein Panel
Publication Type: Abstract Only
Publication Acceptance Date: September 15, 1999
Publication Date: N/A
Technical Abstract: As a result of United States nutrition labeling legislation, i.e., The Nutrition Labeling and Education Act of 1990, there is a need for rapid and accurate methods for the analysis of nutrients in foods. Dietary fiber is one of the components of foods included in the Nutrition Labeling and Education Act, however, measurement of dietary fiber by conventional methods is expensive and time consuming. Near-infrared spectroscopy has been used for the rapid analysis of certain constituents of agricultural products and has been investigated in our laboratory as a rapid method for the determination of dietary fiber. Initially, a data set consisting of cereal food products, e.g., breakfast cereals, crackers, pastas, brans, was used to determine the potential of near-infrared reflectance spectroscopy for the evaluation of dietary fiber. The cereal products were ground and scanned to obtain near-infrared spectra (1100-2500 nm) using a scanning monochromator. Total, insoluble and soluble dietary fiber were then determined using AOAC Method 991.43. Calibrations were developed for the determination of total, insoluble and soluble dietary fiber using modified partial least squares regression. The data set was subsequently expanded to include cereal food products with wide variations in fat, sugar, and moisture content and calibrations developed for dietary fiber prediction. In addition to near-infra-red spectroscopy the potential of combining near-infrared and Raman spectroscopy has been investigated. The results of these studies will be summarized and the feasibility of spectroscopic methods for nutrition labeling purposes will be assessed.