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United States Department of Agriculture

Agricultural Research Service

Title: Characterization of Genes Expressed in the Flooding Tolerant Response of Soybean by Mrna Differential Display Technique.

item Alves, Jose - UNIV FED DE LAVRAS
item Preiszner, Johanna - OHIO STATE UNIVERSITY
item Vantoai, Tara

Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: January 18, 1998
Publication Date: N/A

Technical Abstract: Flooding is a common environmental stress that suppresses plant growth and yield. We have identified soybean genotypes that are tolerant to flooding. When 3-day-old seedlings were submerged for 72 h, seedlings of the tolerant line, Huai 849, survived much better than seedlings of the susceptible line, Nizhen. Huai 849 seedlings that survived also produced more vigorous root and shoot growth during the post-flooding recovery than did Nizhen seedlings. Our objective was to determine the genes associated with flooding tolerance in soybean using mRNA differential display technique (DDRT-PCR). RNAs were isolated from roots of Huai 849 and Nizhen seedlings before and after 72 hours of submergence and reverse transcribed. RT-PCR of the cDNA using 16 independent primer sets produced 960 bands. Forty- three of these bands showed differentially expressed patterns and were classified into one of four classes. Class 1 is present in Huai 849 both before and after flooding, but absent in Nizhen both before or after flooding; Class 2 is present in Huai 849 both before and after flooding, present in Nizhen before flooding, but absent after flooding; Class 3 is only present in Huai 849 after flooding; Class 4 is only present in Nizhen after flooding. These bands were reamplified and used as probes in Northern analyses. Fifteen bands which reproducibly expressed the differential pattern were cloned. The clones were characterized by restriction mapping. Of these, eight clones with unique restriction maps were sequenced. A Genebank search showed no significant homology between six of the eight sequences to known genes. The other two clones showed over 90% homology with a shaggy kinase gene.

Last Modified: 4/18/2015
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