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United States Department of Agriculture

Agricultural Research Service

Title: Cloning and Expression of the Pediocin Operon in Streptococcus Thermophilusand Other Lactic Fermentation Bacteria

Authors
item Coderre, Peter
item Somkuti, George

Submitted to: Current Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 25, 1999
Publication Date: N/A
Full Text Available

Interpretive Summary: The dairy fermentation culture Streptococcus thermophilus enjoys food-grade status since it is ingested together with the food product. The culture is an ideal host for new genes of natural antimicrobial substances such as pediocin, a peptide with inhibitory activity against several foodborne pathogens, including Listeria. By using genetic engineering techniques, several special DNA molecules were constructed for producing the pediocin gene complex in intermediary hosts such as Escherichia coli in larger quantities needed for uptake by selected dairy fermentation cultures. The pediocin gene remained functional in dairy streptococci and lactococci for several generations and the antimicrobial peptide produced inhibited the growth of the foodborne pathogen Listeria. The stable maintenance of the pediocin gene in lactic cultures will allow the development of fermented dairy foods with a built-in bioprotective shield to prevent contamination by Listeria.

Technical Abstract:

Last Modified: 9/2/2014
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