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United States Department of Agriculture

Agricultural Research Service

Title: Variability for Processing Quality Traits in Two Recombinant Inbred Populations of Kidney Bean

Authors
item Posa, Maria - MICHIGAN ST UNIVERSITY
item Hosfield, George
item Kelly, James - MICHIGAN ST UNIVERSITY
item Grafton, K - NO DAKOTA ST UNIVERSITY

Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Proceedings
Publication Acceptance Date: May 15, 1999
Publication Date: N/A

Interpretive Summary: Both dark and light red kidney beans receive scrutiny by processors and consumers for the cooked appearance of individual grains. We conducted an experiment on two populations of kidney beans to see how they varied for processed appearance. Population I was composed of 75 lines which resulted from crossing the varieties, Montcalm and California Dark Red Kidney. Population 2 had 73 unique lines and resulted from crossing Montcalm by California Early Light Red Kidney. The experiment was grown in North Dakota in 1996 and Michigan in 1996, 1997, and 1998. A statistical treatment of data from both populations by a technique called the analyses of variance indicated that for processed bean appearance each line was different from every other line. Differences occurred because of the unique complement of genes in each breeding line that controlled trait expression. Some lines interacted with the environment showing good processed bean appearance in some years but not in others. In Population I, 11 lines had consistently high acceptability scores for appearance. Of these 11 lines, 3 lines consistently appeared in the top 25% of lines in all four environments. In Population 2, 9 lines scored in the top 23% for appearance in three environments and 4 of these lines had superior appearance scores in all 4 environments. Significant variation for processed bean appearance allows the plant breeder to practice directional selection for further improvement of this trait. A knowledge of the way a breeding line reacts to its growing environment both in terms of magnitude and direction (either good or poor) aids the breeder in allocating resources for selection and testing procedures, thus, increasing the efficiency of the breeding program.

Technical Abstract: Processing quality in dry bean (Phaseolus vulgaris L.) is multi-genic trait that has been shown to have considerable variation within and among populations and also across environments. Overall processing quality is affected by the rate of water uptake, hydration coefficient, seed uniformity, texture, degree of clumping and splits, and method of preparation and cooking. Two recombinant inbred populations of kidney bean were developed and evaluated for processing quality. Population I, composed of 75 recombinant inbred lines (RILs), was from a Montcalm/California Dark Red Kidney 82 cross. Population II, with 73 RILs, was from a Montcalm /California Early Light Red Kidney cross. RILs from both populations were planted in North Dakota in 1996 and in Michigan in 1996, 1997, and 1998. Beans of each RIL were thermally processed using established procedures. Appearance and degree of splitting of each sample and the check varieties were scored subjectively on a 1-7 scale to represent the minimum and maximum acceptability levels of the traits, respectively. Genotypes and genotype environment interactions were significant based on analyses of variance. In Population I, eleven lines had consistently high acceptability scores for appearance in three of the four environments. Of these, three lines consistently appeared in the top 25% in all four environments (mean=5.0; range=4.0-6.3). In Population II, nine lines scored in the top 25% for appearance in three environments and four lines had good performance in all environments (mean=4.4; range=3.8-5.7). The processing quality data was used to obtain DNA bulks for bulked segregant analysis in order to screen for molecular markers associated with QTLs controlling canning quality.

Last Modified: 9/22/2014
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