Submitted to: International Symposium on Pollutant Responses in Marine Organisms
Publication Type: Abstract Only
Publication Acceptance Date: March 24, 1999
Publication Date: April 25, 1999
Citation: EVANS, J.J., KLESIUS, P.H. THE USE OF PRIMARY CULTURES OF BRAIN CELLS FROM TILAPIA OREOCHROMIS NILOTICA AND OTHER TELEOST CELLS IN TOXICITY TESTING. TENTH INTERNATIONAL SYMPOSIUM ON POLLUTANT RESPONSES IN MARINE ORGANISMS. 1999. Technical Abstract: Chemical, bacterial, fungal and algal toxins occurring in the aquaculture and natural environments justify the need for rapid, inexpensive and accurate in vitro tests to predict toxicity. Primary cultures of teleost brain cells and cytotoxicity assays have not been previously employed to assess toxicity of these agents. Primary cultures of brain cells from tilapia and the following colorimetric assays, 5-(3-carboxylmethoxy-phenyl -2-(4,5-dimethylthiazoyl)-3-(4-sulfophenyl) tetrazolium, inner salt (MTS), 3-(4,5-dimethylthiazol-2-yl)-2,5-(diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH), bromodeoxyuridine (BrdU) and neutral red (NR) were used to measure cytotoxicity and compared to results from other teleost primary cultures of brain cells and established cell lines. The results showed that primary brain cells were more sensitive than cell lines in predicting aquatic neurotoxicity. Significant positive correlations between MTS and MTT and between these tetrazolium-based assays and LDH were found. The NR assay was the least sensitive and was not positively correlated to the other assays. The study indicated the usefulness of primary cultures of brain cells and cytotoxicity assays to evaluate chemical and bacterial toxicity and their potential uses in the assessment of mycotoxins and biotoxins.