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United States Department of Agriculture

Agricultural Research Service

Title: Production of Bydv Antisera by DNA Immunization

Authors
item Pal, Narinder - UNIV OF ILLINOIS
item Domier, Leslie
item D'Arcy, Cleora - UNIV OF ILLINOIS

Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 16, 1999
Publication Date: N/A

Technical Abstract: We report here the induction of antibodies to the 22-kDa coat protein (CP) of barley yellow dwarf virus strain PAV (BYDV-PAV) by genetic immunization. A cDNA sequence encoding the 22-kDa CP of BYDV-PAV was cloned into a mammalian expression vector (pCDNA22K), entrapped in liposomes and injected intramuscularly into BALB/c mice. To target the BYDV antigen to sites of immune induction and increase its effective dose, the 22-kDa sequence also was fused with that encoding the cytotoxic T-lymphocyte antigen 4 (pCTLA4-22K). Three weeks post-immunization, a specific immune response was detected. Injection with either pCDNA22K or pCTLA4-22K resulted in antibody titers significantly above those of non-immunized control mice. Furthermore, mice injected with pCTLA4-22K showed significantly higher titers than mice injected with pCDNA22K after the initial bleeds. Antibody titers did not increase significantly after the second injection in mice immunized with pCTLA4-22K. However, antibody titers of the two groups of mice after second and third injections were not significantly different. These results demonstrate that DNA immunization is a powerful technique for the production of virus specific antisera and can be readily applied to difficult to purify or rare proteins.

Last Modified: 10/23/2014
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