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United States Department of Agriculture

Agricultural Research Service

Title: Development of Molecular Markers Linked to High Pigment (Hp) and Dark Green(dg) Loci in Tomato

item Zhang, Yiping

Submitted to: American Society of Horticulture Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 1999
Publication Date: N/A

Technical Abstract: The carotenoids have an important influence on tomato fruit quality and enhance the fruit's contribution to human nutrition. Expression of the high pigment (hp) locus in tomato results in increased total carotenoids and increased efficiency of utilization of the polyenes. A similar mutant dark green (dg) contains higher level of chlorophyll in immature fruit and results in darker red pigmentation, both externally and internally in ripe fruits. Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) analyses were performed using two pairs of near isogenic lines (NILs) designed to be isogenic at the hp and dg loci. Sixty-four AFLP primer pairs and more than 1000 RAPD 10-mer primers were screened for polymorphism between each pair of the NILs. One RAPD marker was identified to be linked to the hp gene, and two AFLP primer pairs showed polymorphic fragments which distinguished the dg NILs. The markers identified in this study will be converted to allele specific SCAR (sequence characterized amplified region ) markers which are more useful in marker assisted selection breeding programs.

Last Modified: 4/22/2015
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