Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: May 12, 1999
Publication Date: N/A
Technical Abstract: Previously, we have used an agar plate fluorescence method to identify lipase activity in selected bacterial strains obtained from the NRRL Culture Collection. Now, we have characterized the lipases of more than 50 Bacillus and Pseudomonas strains based on their positional specificity for triglycerides (triolein) for those strains which showed the greatest activity in the original screening. Lipase was produced by growing the cultures in tryptone-glucose-yeast extract medium for 24 hr at 30C before addition of triglyceride. The lipase was allowed to act on the triglyceride for three days before analysis by thin layer chromatography. Of the bacterial strains tested, approximately 70% displayed random specificity and the remainder were 1,3 specific.