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United States Department of Agriculture

Agricultural Research Service

Title: Characterization of Malus Hupehensis (Tea Crabapple) with DNA Simple Sequence Repeats

Authors
item Benson, Laura - CORNELL UNIVERSITY
item Benson, Laura - CORNELL UNIVERSITY
item Zimmerman, Richard
item Zimmerman, Richard
item Lamboy, Warren
item Lamboy, Warren

Submitted to: American Society of Horticulture Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 1999
Publication Date: July 1, 1999
Citation: BENSON, L., ZIMMERMAN, R.H., LAMBOY, W.F. CHARACTERIZATION OF MALUS HUPEHENSIS (TEA CRABAPPLE) WITH DNA SIMPLE SEQUENCE REPEATS. AMERICAN SOCIETY OF HORTICULTURE SCIENCE MEETING. 1999.

Technical Abstract: Simple sequence repeats (SSRs) are highly polymorphic regions of DNA that can be used for the molecular characterization of apple (Malus) germplasm. SSR markers are sufficiently variable to distinguish between individual plants in wild Malus species. In this study, accessions of Malus hupehensis were screened for fragment length variation in PCR amplified simple sequence repeat regions of DNA. The fragment length phenotype produced by five SSR primer pairs showed no variation between two lineages of M. hupehensis collected in the Changjiang (Yangtse) River valley. One lineage was collected by E.H. Wilson in 1908 near the city of Ichang, Hubei Province. The second lineage was collected by cooperators at China's Southwest Agricultural University (SWAU) in 1997 near the city of Chongqing (Chungking). M. hupenhensis Plant Introduction No. 588760 from the National Plant Germplasm System lacks provenance, but displays a fragment length phenotype identical to both the Wilson and SWAU lineages. The sprea of a clone may be aided by asexual reproduction through seed, which is not uncommon in polyploid apples. Two seedlings each of 15 maternal trees from the SWAU lineage were assayed for ploidy level by flow cytometry. The DNA content per nucleus for all SWAU progeny fell within the range for triploids, 2.19 to 2.68 pg DNA/nucleus. It appears that plant explorers in China separated by almost 90 years have succeeded in sampling a single clonal lineage of M. hupehensis.

Submitted to: American Society of Horticulture Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 1999
Publication Date: July 1, 1999
Citation: BENSON, L., ZIMMERMAN, R.H., LAMBOY, W.F. CHARACTERIZATION OF MALUS HUPEHENSIS (TEA CRABAPPLE) WITH DNA SIMPLE SEQUENCE REPEATS. AMERICAN SOCIETY OF HORTICULTURE SCIENCE MEETING. 1999.

Technical Abstract: Simple sequence repeats (SSRs) are highly polymorphic regions of DNA that can be used for the molecular characterization of apple (Malus) germplasm. SSR markers are sufficiently variable to distinguish between individual plants in wild Malus species. In this study, accessions of Malus hupehensis were screened for fragment length variation in PCR amplified simple sequence repeat regions of DNA. The fragment length phenotype produced by five SSR primer pairs showed no variation between two lineages of M. hupehensis collected in the Changjiang (Yangtse) River valley. One lineage was collected by E.H. Wilson in 1908 near the city of Ichang, Hubei Province. The second lineage was collected by cooperators at China's Southwest Agricultural University (SWAU) in 1997 near the city of Chongqing (Chungking). M. hupenhensis Plant Introduction No. 588760 from the National Plant Germplasm System lacks provenance, but displays a fragment length phenotype identical to both the Wilson and SWAU lineages. The sprea of a clone may be aided by asexual reproduction through seed, which is not uncommon in polyploid apples. Two seedlings each of 15 maternal trees from the SWAU lineage were assayed for ploidy level by flow cytometry. The DNA content per nucleus for all SWAU progeny fell within the range for triploids, 2.19 to 2.68 pg DNA/nucleus. It appears that plant explorers in China separated by almost 90 years have succeeded in sampling a single clonal lineage of M. hupehensis.

Last Modified: 9/10/2014
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