|Lopez-Corrales, Nestor - ROSLIN INST, SCOTLAND UK|
|Beattie, Craig - UNIV. MINNESOTA, ST. PAUL|
Submitted to: Cytogenetics and Cell Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 29, 1999
Publication Date: N/A
Interpretive Summary: Fifty-three microsatellites were assigned to 18 different chromosomes. These assignments improve the integration of the physical and genetic maps in specific regions of the genome. Furthermore, from the assignments we can determine the portion of the genome spanned by the genetic map and estimate rates of recombination in specific regions of the genome. This information will be useful in identifying genes of economic importance.
Technical Abstract: This study provides 53 new fluorescent in situ hybridization cytogenetic assignments for microsatellite markers linked on the swine genetic map. Forty microsatellites are physically assigned for the first time. The chromosomal locations of eight markers were either confirmed or refined, while five loci were assigned to locations which differed from previous reports. Markers were selected to provide physical anchors based on their presumed proximity to centromeres or telomeres and at approximately 30 cM intervals across the genetic map. The number of physical anchors for chromosomes SSC 8, 15, and 18 linkage groups was significantly improved. Centromeric regions were localized to areas less than 10 cM for SSC 1, 2, 3, 6, 7, 8, and 9. Although recombination rate was generally higher across small biarmed chromosomes and lowest for large acrocentric chromosomes, two regions with particularly low (1q2.1-2.9 and 13q2.3-4.1) and three regions with extremely high (5p1.2-1.5, 6p1.3-1.4 and 12p1.4-1.5) rates of recombination were detected. These assignments represent an overall increase of 10% in physically assigned markers and more than 20% increase in the number of the Type II loci assigned to the pig cytogenetic map.