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United States Department of Agriculture

Agricultural Research Service

Title: Utility of DNA Fingerprinting to Distinguish Bordetella Avium, Bordetella Hinzii and Alcaligenes Faecalis Isolates (Oral Presentation for the 33rd United States-Japan Natural Resources Mycoplasmosis Mtg. (Ujnr)

Authors
item Sacco, Randy
item Nordholm, Gwen

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 20, 1998
Publication Date: N/A

Technical Abstract: Bordetella avium causes respiratory tract infections in poultry (coryza or rhinotracheitis). Organisms previously referred to as B. avium-like or Alcaligenes faecalis type II have recently been designated as B. hinzii. Although these latter organisms have been isolated from diseased birds, there is little evidence that they are pathogenic. Four isolations of B. hinzii from humans have been reported, including isolations from blood of an AIDS patient and sputum of a patient with cystic fibrosis. The objective of the experiments reported herein was to examine the utility of DNA fingerprinting to distinguish B. avium, B. hinzii, or A. faecalis strains representing isolates from the United States, South Africa, and Germany. Digestion of chromosomal DNA with twenty restriction endo- nucleases (RE) was carried out for fingerprint analysis. The digested DNA fragments were electrophoresed in agarose gels and stained with ethidium bromide. We found that RE digestion with Hinf I produced fingerprint patterns in the 2.0- to 10-kb range and readily distinguished B. avium, B. hinzii and A. faecalis isolates. Furthermore, Hinf I digestion of chromosomal DNA from B. avium strains produced eight distinct fingerprint profiles. DNA fingerprinting of Bordetella isolates may prove useful in epidemiological studies.

Last Modified: 4/17/2014
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