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United States Department of Agriculture

Agricultural Research Service

Title: Mapping of the Bovine Growth Hormone-Releasing Hormone Receptor (Ghrh-R) Gene to Chromosome 4 by Linkage Analysis Using a Novel Pcr-Rflp.

Authors
item Connor, Erin - UNIVERSITY OF MARYLAND
item Ashwell, Melissa
item KAPPES, STEVEN
item Dahl, Geoffrey - UNIVERSITY OF MARYLAND

Submitted to: Journal of Animal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 15, 1999
Publication Date: N/A

Interpretive Summary: Previously we have found that differences among beef bulls in their growth hormone response to injection of growth hormone-releasing hormone (GHRH) are related to differences in growth performance and carcass composition. We believe that these differences are mediated at the GHRH receptor (GHRH-R) and that GHRH-R may be an important candidate gene controlling growth performance and carcass traits of beef cattle. A sequence variant was identified within the GHRH-R gene so it could be placed on the USDA bovine genetic map. The gene was mapped to bovine chromosome 4. The same gene has been mapped to human chromosome 7 and pig chromosome 18. Mapping of the bovine gene to chromosome 4 provides additional evidence that human chromosome 7, pig chromosome 18, and bovine chromosome 4 carry the same genes across different species.

Technical Abstract: Previously we found that differences among beef bulls in their growth hormone response to intravenous injection of growth-hormone releasing hormone are related to differences in growth performance and carcass composition. We believe that these differences are mediated at the GHRH-R and that the gene may be an important candidate gene controlling growth performance and carcass traits in beef cattle. Therefore polymorphisms within the genes could be used to investigate the gene's association with economically important traits. One restriction fragment length polymorphism was identified within a PCR amplification product of the bovine GHRH-R gene using the restriction enzyme Eco57I and was used to map the gene in the MARC mapping reference population. The gene was mapped to bovine chromosome 4 between markers INRA072 and BMS3013, providing additional support to the orthologous relationship among bovine chromosome 4, human chromosome 7, and porcine chromosome 18.

Last Modified: 9/10/2014