Submitted to: Animal Genetics
Publication Type: Research Notes
Publication Acceptance Date: June 1, 1999
Publication Date: N/A
Technical Abstract: A detection test for the Q204X mutation of the bovine myostatin gene was de developed. This test consisted of a PCR reaction follow-up by digestion of the DNA fragment by the restriction endonuclease FnuH 1. The reverse primer used inthe PCR was designed such as there is a cutting site if the "normal" allele is amplified. If the Q204 allele is amplified, the cutting gsite is eliminated. This test is faster and less expensive to perform tha sequencing PCR products, which was previously the only way to resolve the two alleles.