|Westgate, Mark - IOWA STATE UNIVERSITY|
|Orf, J - UNIVERSITY OF MINNESOTA|
Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 22, 1998
Publication Date: N/A
Technical Abstract: Soybean genotypes vary widely in seed protein accumulation in response to temperature. The profiles of storage protein accumulation in the mature seeds were examined to determine if specific protein fractions were responsible for the observed changes in total seed protein. Three genotypes, Evans, proto and PI 132.217, which are known to vary in protein response to temperature, were grown at 20/12 deg C, 27/20 deg C, or 35/27 deg C during seed filling and maturation. Proteins were extracted from mature seeds and analyzed by SDS-PAGE. Scanning densitometry of stained gels indicated major differences in band intensities among the genotypes and across temperatures within genotypes. In Evans, a temperature responsive genotype, glycinin subunit levels increased with temperature. In PI113.217, which maintained seed protein concentration across temperatures, compensation between conglycinin and glycinin subunits was evident. Overall, increased protein accumulation was associated with greater glycinin subunit production.