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Title: CHARACTERIZATION OF A ZETA CLASS GLUTATHIONE S-TRANSFERASE GENE IN WHEAT

Author
item YE, ZHAOHUI - DEPT HORTICULTURE, UMCP
item SUBRAMANIAM, KUPPUSWAMY - DEPT HORTICULTURE, UMCP
item SHANER, G - BOT. & PLANT PATH, PURDUE
item SOLOMOS, T - DEPT HORTICULTURE, UMCP
item Ueng, Peter

Submitted to: Plant Molecular Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/30/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary: Plant glutathione-S-transferases (GSTs) play important roles in cell metabolism and reportedly respond to herbicide application and disease infection. They can detoxify the herbicides, induce acquired systemic disease resistance and cause tissue senescence in plants. We have identified a gene encoding the GST enzyme in wheat. It has high homology with the carnation GST at the molecular level. It may be regulated by ethylene and cause tissue senescence. The difference in gene structure in many wheat cultivars implies the usage of this gene as genetic marker in plant breeding. The information from this research leads to a better understanding of disease resistance and to developing resistant wheat in agriculture.

Technical Abstract: Plant glutathione S-transferases (GSTs) play important roles in the detoxification of toxic compounds. A GST gene, TAGST, was cloned from wheat by RT/PCR differential display method. It has different substrate specificities with two wheat GSTs reported previously. The nucleotides and amino acids sequences have high homology with carnation GST Tgene SR8 and human GST gene GSTZ1, which suggests it belongs to a newly proposed GST class Zeta. A polymorphism of the 3' untranslated region of TAGST gene in different wheat cultivars was also discovered. Sixteen wheat cultivars studied have a 89bp additional sequence in the 3' untranslated region, while the other 5 cultivars do not have. Though the biological importance of this polymorphism is unknown, it can be used as a genetic marker in wheat breeding.