ARS | Publication request: A RAPD MARKER WITH A HETEROZYGOUS FORM LINKED TO THE RSV1 LOCUS IN SOYBEAN

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 1, 1998
Publication Date: N/A

Technical Abstract: Soybean mosaic virus (SMV) is a widely occurring, seed-transmitted disease. The objective of this research was to identify RAPD markers linked to loci conditioning resistance to SMV using near-isogenic lines with Williams as the recurrent parent and PIs 96.983, 360.844 and 486.355 as the donor parents. These introductions are all known to contain a resistance allele at the Rsv1 locus. After screening over 250 random primers, a 1000 bp RAPD fragment designated OPN-111000 was identified to be polymorphic between these SMV resistant and susceptible lines. Characterization of the F2 and F3 populations of crosses between Williams and the near-isogenic lines developed from PIs 360.844 and 486.355 confirmed a repulsion phase linkage between OPN-111000 and Rsv1 at a distance of 1.2 cM. Thirty-five ancestors of modern U.S. cultivars as well as selected current cultivars with known reaction to SMV were characterized with this marker. Plants heterozygous for Rsv1 have a much less intense OPN-111000 fragment than those plants that are homozygous for Rsv1. This reduced intensity was confirmed in the F1, F2, and F3 generations.