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United States Department of Agriculture

Agricultural Research Service

Title: Continuous Callus Production and Regeneration of Garlic (Allium Sativum L) Using Root Segments from Shoot Tip-Derived Plantlets

item Myers, J Michele - DEPT OF HORT UW MADISON
item Simon, Philipp

Submitted to: Plant Cell Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 30, 1998
Publication Date: N/A

Interpretive Summary: Garlic is a crop which grows slowly in the field and also grows slowly in tissue culture, a system for plant propagation in test tubes. In this experiment we developed a system for reliably growing garlic in tissue culture by carefully varying the plant growth hormones used to grow these garlic plants. We were able to grow complete plants four months after starting the tissue culture. This research has a potential positive impact for garlic tissue culture researchers.

Technical Abstract: Root segments from shoot tip-derived plantlets of the garlic (Allium sativum L.) Clones DDR7099, PI38319, and Piacenza' were utilized as an explant source for continuous, friable callus production. The best callus production occurred on root segments initially cultured on medium with 2,4-dichlorophenoxyacetic acid (2,4-D)(4.5 uM) for 8 weeks, then subcultured to meduim with 4-amino-3,5,6 trichloropicolinic acid (picloram) (4.7 uM) + 6-(y-y-dimethylallylamino) purine (2iP) (0.49 uM) for 8 weeks. Embryogenic, friable callus was transferred to liquid suspension medium for one month and then transferred to solid regeneration medium for 14 weeks. The best shoot and root regeneration (85.3% and 35.8%, respectively) occurred on 4 month old calli from the clone DDR7099. In all clones, regeneration rate decreased as callus age increased.

Last Modified: 4/22/2015