|Vivek, B S - DEPT OF HORT UW MADISON|
Submitted to: Journal of American Society of Horticulture Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 24, 1998
Publication Date: N/A
Interpretive Summary: Carrots are related to several wild plant species of the genus Daucus, but it is not known how close these relationships are, based upon plant morphology. In this study we used an evaluation of DNA from carrot and its wild relatives to better understand their relationships. Using DNA from the nucleus of these plants we found that relationships estimated form DNA matches quite well with relationships based upon plant morphology. A few exceptions were noted and these are being studied further. This research has a potential positive impact for carrot geneticists and breeders attempting to intercross carrots.
Technical Abstract: Current classifications of the genus Daucus are based on morphological and anatomical characteristics. We have used single to low copy nuclear restriction fragment length polymorphisms (nRFLPs) to describe the phylogeny and relationships of eight Daucus species including cultivated carrot (D. Carota L.). Parsimony analysis of 247 characters (DNA fragments from 58 probe-enzyme combinations) yielded a tree in which accessions were grouped into three major clades and phenetic analysis using Jaccard's coefficient yielded two major clusters. The Phylogenetic relationships from the nuclear RFLP data generally agreed with an earlier morphological classification. Resolution and placement of D. guttatus and D. muricatus were not consistent with the morphological classifications. Molecular variation among carrot inbreds was large.