|Dominguez, J - INIA, MADRID, SPAIN|
|Ezquerra, A - ANIMAL INIA,MADRID,SPAIN|
|Alonso, F - ANIMAL INIA,MADRID,SPAIN|
|Mccullough, K - INST.OF VIROL.,SWITZERLAN|
|Summerfield, A - INST.OF VIROL.,SWITZERLAN|
|Bianchi, A - CEN.VET.INST.,NETHERLANDS|
|Zwart, R - CEN.VET.INST.,NETHERLANDS|
|Kim, Y - CHICAGO MEDICAL SCHOOL|
|Blecha, F - KANSAS STATE UNIVERSITY|
Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 30, 1998
Publication Date: N/A
Interpretive Summary: Understanding of the porcine immune response is frequently limited by the availability of reagents to adequately examine immune cell populations. Monoclonal antibodies (mAb) are antibodies that target distinctive areas on a cell and are a powerful tool allowing us to observe changes in the white blood cell populations. Forty-five mAbs submitted to the Second International Swine CD workshop were analyzed by six different laboratories. Several antibodies were thus able to be defined for future use to differentiate a mature immune cell (macrophage) from its precursors. Furthermore, several mAbs were identified that allows differentiation of three subsets of white blood cell population. The mAbs that were characterized will allow researchers or veterinarians to identify cell population changes and the stages of maturation and activation of immune cells in pigs. Having more immunological tools, like these mAbs, may yallow us to identify early indicators of stress or disease in pigs.
Technical Abstract: Forty-five mAbs submitted to the Second International Swine CD Workshop were analyzed by six different laboratories for their possible reactivity with porcine myelomonocytic cells using flow cytometry and immunohistochemistry. As a result of these analyses, a new swine workshop cluster, SWC9, composed of two mAbs that recognize an antigen selectively expressed on mature macrophages, was defined. In addition, several mAbs were identified, allowing the differentiation of granulocytes from monocytes/macrophages, or monocytes from macrophages. Further work is required to identify the antigen recognized by these mAbs. Nevertheless, they should already prove useful for the identification of different stages in the macrophages maturation/differentiation, and will certainly aid analyses on the complexity of the mononuclear phagocyte system in the pig. Finally, the cross-reactivity of three anti-human CD14 mAbs with porcine myelomonocytic cells was established in this workshop.