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ARS Home » Research » Publications at this Location » Publication #94305
Title: ENDOTOXIN CHALLENGE DIFFERENTIALLY AFFECTS CYTOKINE PRODUCTION AND METABOLIC STATUS OF GROWING AND FINISHING SWINE

Author
item MYERS, MICHAEL - US FDA, LAUREL, MD
item FARRELL, DOROTHY - US FDA, LAUREL, MD
item BAKER, JOHN - US FDA, ROCKVILLE, MD
item COPE, CAROL - US FDA, LAUREL, MD
item Clover, Christina
item Steele, Norman

Submitted to: Domestic Animal Endocrinology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/19/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary: Growing and finishing swine challenged with endotoxin at a dose of either 2 20 ug/kg body weight did produce a known immune response - that is, TNF i dose-response relationship as measured by bioassay. Peak TNF plasma leve were observed 1-2 hours post challenge, returning to baseline values 4 hour post-challenge. There was no effect of endotoxin dose or metabolic status. .Endotoxin-induced decreases in blood glucose levels were primarily dose-de t; although the metabolic status of the animals also affected glucose levels, with growing animals exhibiting greater sensitivity compared to finishing animals. In contrast, changes in plasma urea nitrogen and free f acid (FFA) levels were strictly related to the metabolic status. Urea nitr levels were unchanged in growing swine, whereas they were increased in fini swine and remained elevated 24 hours post challenge. FFA levels in growing finishing swine increased 3-6 hours post challenge. FFA levels returned to obasal values for finishing swine 24 hours post challenge, but in growing s remained elevated 24 hours post challenge. These results demonstrate that endotoxin challenge of swine result in a pattern of changes that are depend upon both dose of endotoxin used and the metabolic status of the animal examined.

Technical Abstract: Growing and finishing swine challenged with endotoxin at a dose of either 2 or 20 ug/kg body weight did produce a known immune response - that is, TNF in a dose-response relationship as measured by bioassay. Peak TNF plasma levels were observed 1-2 hours post challenge, returning to baseline values 4 hours post-challenge. There was no effect of endotoxin dose or metabolic status. Endotoxin-induced decreases in blood glucose levels were primarily dose-dep t; although the metabolic status of the animals also affected glucose levels, with growing animals exhibiting greater sensitivity compared to finishing animals. In contrast, changes in plasma urea nitrogen and free fatty acid (FFA) levels were strictly related to the metabolic status. Urea nitrogen levels were unchanged in growing swine, whereas they were increased in finishing swine and remained elevated 24 hours post challenge. FFA levels in growing and finishing swine increased 3-6 hours p challenge. FFA levels returned to basal values for finishing swine 24 hours post challenge, but in growing swine remained elevated 24 hours post challenge. These results demonstrate that endotoxin challenge of swine result in a pattern of changes that are dependent upon both dose of endotoxin used and the metabolic status of the animal examined.