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United States Department of Agriculture

Agricultural Research Service

Title: Comparison of the Monoglucosylceramides of Caenorhabditis Elegans and Meloidogyne Incognita

Author
item Chitwood, David

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: August 13, 1998
Publication Date: N/A

Technical Abstract: Despite putative structural and bioregulatory roles, the glycolipids of nematodes have received much more limited investigation than other types of lipids. Monoglycosylceramides are among the most simple glycolipids and consist of a single sugar residue attached to a long chain sphingoid base connected to a fatty acid via an amide linkage. These glycolipids were purified from nematode extracts with HPLC and comprised 0.17% of the dry weight of both Caenorhabditis elegans and Meloidogyne incognita. The structures of the compounds were elucidated with mass spectrometry, nuclear magnetic resonance spectroscopy, and analysis of methanolysis products of the parent glycolipids via GC-MS of appropriate derivatives. The glycosylceramides from each species were unusual in that the only long chain base detected was an iso-branched, 17-carbon compound with a C-4 double bond (15-methyl-2-aminohexadec-4-en-1,3-diol). Glucose was the only sugar moiety detected. The fatty acids of the C. elegans material consisted primarily of a series of primarily straight-chain, saturated, 2-hydroxylated C-20 to C-26 acids; some iso-branched analogs also occurred. In M. incognita, however, the monoglucosylceramide fatty acids were largely unsaturated, with nearly two-thirds being a hydroxylated 24-carbon monounsaturated acid (2-hydroxy-n-tetracosenoic acid)

Last Modified: 8/21/2014
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