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United States Department of Agriculture

Agricultural Research Service

Title: Biocatalytic Production of Hydroxy Unsaturated Fatty Acids

Author
item Hou, Ching

Submitted to: Japan Oil Chemists' Society Meeting
Publication Type: Abstract Only
Publication Acceptance Date: September 17, 1998
Publication Date: N/A

Technical Abstract: It is important to convert vegetable oils and animal fats into value-added industrial products. One of such products is oxygenated fatty acid. Oxygenated metabolites of fatty acids also play a variety of important roles in biological systems. We have been investigating the production of monohydroxy and multihydroxy fatty acids from vegetable oils and their component fatty acids through biocatalysis. Microbial enzymes can produce not only mono-hydroxy but also di- or tri-hydroxy fatty acids. We discovered that a new microbial isolate, Pseudomonas aeruginosa PR3, produced 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) from oleic acid and another new microbial isolate, Clavibacter sp ALA2, produced 12,13,17- trihydroxy-9(Z)-octadecenoic acid (THOA) from linoleic acid. We also found other products such as tetrahydrofuranyl unsaturated fatty acids in this THOA system. These products were isolated and their structures determined. .The structures of these hydroxy unsaturated fatty acids resemble those of plant self-defense substances. This is the first report on microbial production of trihydroxy unsaturated fatty acids. On anti-pathogens activity test, we found that at 200 ppm concentration, DOD inhibited the growth of C. albican but did not inhibit the growth of A. flavus, A. fumigatus, C. neoformans and M. infracellulare. We also found that at 200 ppm concentration, THOA inhibited growth of some plant pathogenic fungi: such as wheat powdery mildew and wheat leaf rust.

Last Modified: 10/26/2014
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