|Jim, Wei - IOWA STATE UNIVERSITY|
|Horner, Harry - IOWA STATE UNIVERSITY|
Submitted to: Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 30, 1999
Publication Date: N/A
Interpretive Summary: Enzymes are proteins that help in plant and animal growth and development. One enzyme called beta-1,3-glucanase is involved in male development, embryo growth, germination, pathogen infection, and several other plant processes. We identified 12 classes of this enzyme in soybean. One class expressed in the male (anther) is believed to be associated with a male- sterile trait. (The female is fully fertile). The beta-1,3-glucanases were distributed in the soybean in clusters and two of them were located on the same positions or lanoun fungal resistant traits. Our research results will facilitate genetic engineering of male-sterile soybean, and of disease resistant soybean. Seeds from these beans have the potential for increased yields for soybean growers.
Technical Abstract: Oligonucleotide primers designed for conserved sequences from coding regions of beta-1,3-glucanase genes from different species were used to amplify related sequences from soybean (Glycine max (L.) Merr.]. Sequencing and cross hybridization of amplification products indicated that at least 12 classes of beta-1,3-glucanase genes exist in soybean. Members of classes mapped to 34 loci on five different linkage groups using an F2 population of 56 individuals. Beta-1,3-glucanase genes are clustered onto regions of five linkage groups. Data suggest that more closely related genes are clustered together on one linkage group or on duplicated regions of linkage groups. Northern blot analyses performed on total RNA from root, stem, leaf, pod, flower bud, and hypocotyl, using the different classes of beta-1,3-glucanases as probes, revealed the accumulation pattern of beta-1,3-glucanase mRNA in these organs.