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United States Department of Agriculture

Agricultural Research Service

Title: Combined Aflp and Rflp Mapping in Two Hexaploid Oat Recombinant Inbred Populations

Authors
item Jin, Hua - UNIV OF ILLINOIS
item Domier, Leslie
item Kolb, Frederic - UNIV OF ILLINIS
item Brown, Charles - UNIV OF ILLINOIS

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: July 13, 1998
Publication Date: N/A

Technical Abstract: The segregation of AFLP markers was analyzed in two different hexaploid oat recombinant inbred populations. The first population was derived from a cross of Kanota x Ogle and consisted of 71 recombinant lines, which were previously used to produce an RFLP map of the hexaploid oat genome. The second population was derived from a cross of Clintland 64 and IL 86-5698, barley yellow dwarf virus sensitive and tolerant oat lines, respectively. One hundred twenty-six lines of this population were used to identify loci contributing to tolerance to barley yellow dwarf virus infection. More than 300 AFLP markers were scored in each of the populations, of which 85 could be scored in both populations. These markers were linked to RFLP markers in 20 of 38 Kanota x Ogle RFLP linkage groups. The addition of the 306 AFLP markers to the Kanota x Ogle RFLP data set reduced the number of linkage groups to 33 at a LOD of 8.0 and a recombination fraction of 0.3. Most of the markers that showed distorted segregation in one population were more normally distributed in the other. All but eight of the AFLP markers scored in the Kanota x Ogle population were linked to previously mapped RFLP markers. Thirty six linkage groups were observed in the Clintland 64 x IL 86-5698 population. Two of the linkage groups from this population were consolidated by comparing the segregation of markers in both populations. Markers were linked in similar orders in the two populations and showed a very similar distribution to that of the RFLP markers.

Last Modified: 8/1/2014
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