|Bird, David - DEPT PLANT PATH, NC STATE|
|Opperman, Charles - DEPT PLANT PATH, NC STATE|
Submitted to: Society of Nematology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: July 15, 1998
Publication Date: N/A
Technical Abstract: Developmentally arrested infective juveniles of plant-parasitic nematodes are functionally similar to Caenorhabditis elegans dauer larvae, and may use similar molecular mechanisms to maintain arrest and resume development. Several C. elegans daf genes controlling dauer formation have been identified, including TGF-beta (daf-7) and TGFB-receptor (daf-4, daf-1) signalling molecules. Degenerate primers corresponding to conserved domains of the C. elegans daf-7 gene were used to PCR-amplify putative daf-7 homologs from Heterodera glycines genomic DNA. Full-length clones will be characterized and the genes mapped in the SCN genome. To functionally characterize plant-parasite daf genes, their expression profiles will be compared with C. elegans homologs, and the genes will be tested for complementation of C. elegans daf mutants.