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Submitted to: Association Official Analytical Chemists Midwest Section Program Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 6/10/1998 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Currently DON is separated from foodstuffs and detected using numerous analytical methods including immunoassay and chromatographic techniques such as gas chromatography, thin layer chromatography, and high performance liquid chromatography (HPLC). Existing HPLC methods have relied upon the UV absorption of DON at 220 nm. In the present report, wheat was extracted with acetonitrile/water (84+16 v/v) and the DON was isolated using commercially available solid phase extraction columns. After drying-down the purified extract under nitrogen, the DON was reacted with dansyl hydrazine to form a fluorescent derivative. The derivatized DON was separated and quantified using capillary zone electrophoresis with laser-induced fluorescence detection (CE-LIF). As little as 2.4 pg was quantified when 12 nL of DON standard solution was injected (signal to noise ratio = 22). In spiked wheat samples, DON was detected at 0.1 ug/g (ppm). Recovery of 0.5 ppm DON from spiked wheat samples averaged 88% (SD=15, n=7) while recovery of 1 ppm DON averaged 85% (SD=7, n=12). The results suggest that CE-LIF can be used as an alternative to existing chromatographic methods for the determination of DON in wheat. |
