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United States Department of Agriculture

Agricultural Research Service

Title: Selection of Cryoresistant Variants from a Tn-5 Mutagenesis Library of the Dry Rot Antagonist Enterobacter Cloacae S11:t:07

item Van Cauwenberge, James
item Schisler, David
item Boehm, M - OHIO STATE UNIV
item Bothast, Rodney

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: June 23, 1998
Publication Date: N/A

Technical Abstract: Potato dry rot, caused by Gibberella pulicaris, is a major concern in tuber storages. Developing a cryoresistant variant of Enterobacter cloacae (S11:T:07 NRRL 21050), a dry rot antagonist, would improve the economic feasibility of commercial production. A Tn-5 mutagenesis library of more than 5000 clones was pooled and the mixed population grown in liquid culture. The culture was freeze dried in spent broth using severe conditions (secondary drying to 25 deg C). Samples were stored at 25 deg C and survivors monitored over time. When survivor counts had dropped to less than 10, surviving clones were saved for further tests. A total of 27 surviving clones from multiple experiments were individually retested for increased cryoresistance compared to the parental strain without the TN-5 insert. Selected clones occasionally survived better than the parental culture. Identification of genes associated with increased cryoresistance could benefit the commercial development of many Gram-negative biocontrol agents.

Last Modified: 4/22/2015
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