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United States Department of Agriculture

Agricultural Research Service

Title: Evaluation of Maize Streak Germinivirus (Msv) Resistance in Maize (Zea Mays) Germplasm by Vascular Puncture Inoculation (Vpi)

Authors
item Changa, C - OSU-OARDC
item Louie, Raymond
item Gordon, D - OARDC

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 8, 1998
Publication Date: N/A

Technical Abstract: VPI of maize kernels with MSV gave initial low transmission rates of 1% to 5%. Using variously modified VPI protocols and the MSV susceptible Pioneer Hybrid 3379, transmission rates were increased but were variable ranging from 26.7% to 84.3%. A consistent transmission rate of 69.4 +/- 1.9% was obtained using a protocol involving: 1) MSV inoculum from infected, young maize leaves harvested 12 days post inoculation (dpi); 2) inoculation on both sides of the embryo axis; and 3) a 4 hr inoculation delay after soaking kernels in water. The modified VPI was used to evaluate MSV tolerant (TZi4) and moderately (Longe1) and highly resistant (CIMMYT-bred OSU23i) maize germplasm. Streak symptoms on the tolerant and moderately and highly resistant genotypes attenuated over time so that after 28 dpi, VPI results matched those previously reported for leafhopper inoculations. It was concluded that evaluation of MSV resistance in maize by VPI gave comparable results to those of leafhopper inoculations.

Last Modified: 10/23/2014
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