Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: In Vitro Production of Baculoviruses:selection of Cell Lines to Optimize Yields

Authors
item Vaughn, James
item Clay, Deming - UNIVERSITY OF MD
item Lynn, Dwight
item Shapiro, Martin
item HACKETT, KEVIN

Submitted to: Society for Invertebrate Pathology Meeting
Publication Type: Proceedings
Publication Acceptance Date: April 2, 1998
Publication Date: N/A

Interpretive Summary: NO ORIGINAL DATA GENERATED. INTERPRETIVE SUMMARY NOT REQUIRED

Technical Abstract: Baculoviruses are considered a potentially important tool for managing insect pests and cell culture a desired means of producing them. However, cell culture systems capable of producing the large amount of virus needed at a competitive cost have not been developed yet despite considerable research effort to do so. The cell line used in any in vitro system is an important element both in terms of the quality and the quantity of the final product. The selected cell line must replicate the virus, producing both high yields of budded virus and of polyhedra with high specific activity. The cell line also must be capable of growth in suspension in large volumes. If the virus has been genetically engineered and expressed protein is the desired product, the protein produced should be as similar to the natural protein as possible, including necessary post-translation processing and modification. Most of the reported research has been with four cell lines, Tn-368, BTI-TN-5B1-4, IPLB-Sf-21 or a clone of this line, Sf-9. The AcNPV replicates in approximately 35 cell lines derived from a number of different insect species, life stages and tissues. This variety of cell material has not been exploited in the production of foreign proteins in the baculovirus system, but preliminary studies indicate benefits could be obtained from the use of other cell lines. Results which illustrate some of the benefits and the results of studies to select cell lines for the production of gypsy moth NPV and wild type and engineered AcNPV are discussed.

Last Modified: 9/10/2014