ARS | Publication request: PCR-RFLP OF RDNA IN HETERODERA SPP. AND H. GLYCINES RACES

Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: June 20, 1998
Publication Date: N/A

Technical Abstract: The variability of ribosomal DNA region of eight Heterodera glycines races and the species H. lespedezae, H. schachtii, and H. trifolii were assessed by digestion of PCR amplified ribosomal DNA fragments with 14 restriction endonucleases. The pattern produced by PmaC I differentiated H. schachtii from H. glycines, H. trifolii, and H. lespedezae. Ban I separated H. glycines races 2, 3, and 4 from races 1, 5, 6, 9, and 14 by absence of a 750 bp band in races 2, 3, and 4. Within the group of races, 2, 3, and 4, Hae III differentiated race 2 from races 3 and 4 by the presence of a 680 bp band and absence of two other bands (a 200 bp and a 480 bp band) in race 2. PmaC I further separated race 4 from race 3 by leaving an uncut 1280 bp band in race 4, but not in races 3. Therefore, H. glycines races 2, 3, and 4 can be differentiated from races 1, 5, 6, 9, and 14 digestion with Ban I, and from each other by digestion with Hae III and PmaC I. Juveniles from 10 different cysts within races 1, 2, and 3 demonstrated that the race diagnostic restriction patterns were highly stable.