Author
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Carta, Lynn |
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Submitted to: Society of Nematologists Proceedings
Publication Type: Abstract Only Publication Acceptance Date: 6/20/1998 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Longevity, fecundity and bacterial preference tests on agar plates are being made with a phylogenetically diverse group of bacterial-feeding nematodes found in agricultural soils. The nematodes Caenorhabditis elegans N2, Pristionchus pacificus PS312, Zeldia punctata PS1153, Panagrellus redivivus PS1163, Mesorhabditis sp. PS1179, Oscheius myriophila DF5020, Diploscapter sp. PS2123, and Operculorhabditis sp. LKC 10 have been tested for their preference for E. coli OP-50 (or Enterobacter cloacae 501R3) compared to biocontrol bacteria (Burkholderia cepacia strains JED-2, M-35, Bc-F, Bc-2, Pseudomonas fluorescens, PF5 and SE59, Stenotrophomonas maltophila 34S1, Stenotrophomonas corrugata SB45, and Serratia marcescens, N4-5). Responses to three entomopathogenic bacteria are also reported. Preference for toxic bacteria, or aversion to nutritious bacteria, is not uncommon. Toxicity of Burkholderia cepacia strains is significant for all nematodes except for members of the Mesorhabditinae. Patterns such as this may have significant consequences in field situations with different soil bacteria and nematode communities. Each bacterium is also tested for ability to affect the PGP-3 toxin transporter with C. elegans N2 and pgp-3 strains. The pgp-3 gene from nematode strains especially sensitive to a bacterial toxin can be sequenced to determine functionally and phylogenetically important molecular sites in target (parasitic) and non-target (free- living) nematodes. Bacteria were supplied by Dan Roberts, Dawn Gouge, Kevin Thorpe, USDA, and Creg Darby, University of Washington |
