Submitted to: Beltsville Agricultural Research Center Symposium
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 1998
Publication Date: N/A
Technical Abstract:
Three tomato breeding lines, 97L63, 97L66, and 97L97 which produce fruit high in beta-carotene content have been developed for use in processing applications. These lines were developed from an interspecific cross between L. esculentum cv. FM6203 and the wild tomato relative L. cheesmanii f. minor, accession LA317. LA317 was the donor parent for the Beta allele which conditions accumulation of high beta-carotene levels relative to other colored carotenoids in ripe fruit. Plants are compact, determinate in growth habit, and produce a concentrated set of firm, uniform ripening, crack resistant fruit. Populations constructed during the development of these lines were utilized to identify PCR-based molecular markers linked to the Beta locus. Sixty-four AFLP primer pairs and 1018 arbitrary RAPD primers were utilized to screen these populations for unique polymorphic products which cosegregated with the Beta locus. Two RAPD markers identified in the interspecific L. esculentum x L. cheesmanii f. minor F2 population and one AFLP marker identified in the NIL were confirmed to be tightly linked with the Beta phenotype. RAPD and AFLP products have been cloned for use in mapping and for development of SCAR markers.
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