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United States Department of Agriculture

Agricultural Research Service

Title: Fungal Metabolites Toxic to the Root-Knot Nematode, Meloidogyne Incognita

item Nitao, James
item Meyer, Susan
item Chitwood, David

Submitted to: Society of Nematologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: June 6, 1998
Publication Date: N/A

Technical Abstract: Fungi isolated from soybean cyst nematode eggs collected in China were tested for nematicidal activity. In vitro bioassays of filtered culture broths from these fungi revealed several Fusarium species that appear to secrete metabolites capable of inhibiting Meloidogyne incognita egg hatch. Bioassay-directed isolation of these metabolites was undertaken for one of these fungi, Fusarium equiseti. Culture broth was extracted with XAD-16 gel adsorbent. The XAD gel was washed with water and eluted with methanol. The dried methanol eluate was redissolved in water, sterile filtered, and assayed for its effects on M. incognita egg hatch. Fungal broth extract was found not only to inhibit egg hatch but also to immobilize second stage juvenile larvae that did hatch, confirming that the fungus secretes biologically active metabolites

Last Modified: 4/22/2015
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