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United States Department of Agriculture

Agricultural Research Service

Title: Comparison of the Current Regulatory Hplc Method and Immunoassay Detection Method of the Coccidiostat Halofugione in Chicken Livers from Commercial Packing Plants

item Beier, Ross
item Stanker, Larry

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 6, 1998
Publication Date: N/A

Technical Abstract: The quinazolinone, halofuginone (Hal), is a feed additive used worldwide as an antiprotozoal drug to prevent coccidiosis in commercial poultry (Chickens and Turkeys) production. Hal is a halogenated analogue of the naturally occurring quinazolinone alkaloid febrifugine, and was among the top six coccidiostats used in poultry production in the U.S. from 1985 to 1990. The U.S. poultry coccidiostat market increased from 78.7 million dollars in 1985 to 87.5 million dollars in 1990. The U.S. market was estimated to be 100 million dollars in 1993. Hal was again estimated as being one of the top used coccidiostats in 1995-96, having an approximate U.S. market share of 5-10%. The worldwide total coccidiostat market was estimated to be 500 million in 1995-96. The current regulatory method for Hal utilizes a long digest and chemical extraction procedure followed by HPLC analysis. A quick and economical competitive enzyme-linked immunosorbent assay (cELISA) method for determining Hal residues in chicken liver tissue has been developed. A cELISA screening procedure would drastically improve sample throughput, decrease the cost per sample and the overall time required to analyze the sample. The cELISA also can drastically decrease organic solvent wastes. During 1997 a large-scale evaluation of the cELISA method vs. HPLC method for determining the Hal levels in chicken liver samples was undertaken. The comparison analysis was performed on chicken liver samples obtained from commercial packing plants sent to FSIS for evaluation of potential Hal residues. Both the cELISA method and the HPLC method analyzed a total of 482 samples. This final data set will be presented and evaluated.

Last Modified: 4/22/2015
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