Technical Abstract: Germplasm cannot be fully utilized until it is evaluated for useful traits. The objective of this study was to screen seventy-nine day-neutral primitive accessions of cotton, Gossypium hirsutum L., for resistance to the southern root-knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood race 3. The southern root-knot nematode is a major pest of cotton. Cotton lines were evaluated in a greenhouse test for egg-mass production after being grown for forty days in pots inoculated with nematode eggs. None of the lines produced as few egg-masses as our highly resistant check M315 RNR. Twenty-four lines had significantly fewer egg-masses than highly susceptible M8, an indication they carried an intermediate level of resistance. Germplasm evaluations, such as this, will aid its utilization in breeding programs.