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United States Department of Agriculture

Agricultural Research Service

Title: Use of 16s Rdna Sequences to Identify Predominant Bacteria from Porcine Feces and Waste Handling Pits

Authors
item Whitehead, Terence
item Cotta, Michael

Submitted to: Microbial Ecology International Symposium
Publication Type: Abstract Only
Publication Acceptance Date: August 14, 1998
Publication Date: N/A

Technical Abstract: Storage of swine waste is associated with the production of a variety of odorous compounds, including ammonia, organic acids and alcohols, and sulfides. Although the generation of these chemicals is the result of microbiological activity, little is known about the types of microorganisms responsible for their production. We have initiated an approach to determine the predominant bacterial population present in both pig feces and waste storage pits. Total DNA was isolated from these ecosystems and DNA sequence analyses of PCR amplified 16S rDNA genes was carried out. Similarity analyses of the 16S sequences indicated the presence of primarily low G+C Gram-positive bacteria in both ecosystems. Such genera as Paenicacllus, Facklamia, and Macrococcus were identified from the porcine feces DNA, whereas the pit DNA indicated the presence of Listeria, Pseudomonas, Facklamia, Clostridium, and other genera.

Last Modified: 4/23/2014
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