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United States Department of Agriculture

Agricultural Research Service

Title: Do Different Host Components Alter the Secretory Proteins of Hemipterans

Authors
item Habibi, J - UNIV OF MISSOURI
item Backus, E - UNIV OF MISSOURI
item Coudron, Thomas
item Brandt, S - UNIV OF MISSOURI

Submitted to: Molecular Insect Science International Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: February 28, 1998
Publication Date: N/A

Technical Abstract: Two homopterans, Empoasca fabae and Empoasca abrupta, one phytophagous heteropteran, Lygus hesperus and one entomophagous heteropteran, Podisus maculiventris, were used in this study. A comparison of the proteins from saliva of these insects was conducted in order to better our understanding of salivary components that are involved in feeding by hemipterans. Insects were reared on a primary host and then were pre-test acclimated on a secondary host and/or an artificial diet. Saliva was collected by feeding these species on a sucrose-agarose medium. The proteins in the saliva were separated by size and charge using electrophoretic techniques under denaturing conditions. Electrophoretic analyses of secretory proteins for E. Fabae showed different banding patterns for insects fed on primary or secondary host, or artificial diet. A greater number of proteins were observed in saliva from insects fed on primary hosts versus secondary hosts and artificial diet. Furthermore, the percentage composition of certain proteins increased in the saliva of insects that were fed on the primary host. In contrast, a greater number of proteins were observed for E. abrupta that were fed artificial diet vs. primary host. A greater number of proteins were also observed for artificial diet- reared L. hesperus when secondarily fed on cotton and pinto bean. By comparison, there was minimal difference in the number and percentage of composition of proteins secreted by P. maculiventris fed on three natural hosts and an artificial diet.

Last Modified: 7/28/2014
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