ARS | Publication request: IDENTIFICATION OF AFLP MARKERS ASSOCIATED WITH A HYPERNODULATION MUTANT GENE IN SOYBEAN

Submitted to: Plant Physiology Supplement
Publication Type: Abstract Only
Publication Acceptance Date: June 29, 1998
Publication Date: N/A

Technical Abstract: The hypernodulating characteristic of mutant soybean [Glycine max (L.) Merr.] lines derived from cv. Williams is genetically controlled by a single recessive gene (rj7) which is allelic to the nts-1 gene in cv. Bragg-derived mutants. Previous analysis of one PCR-converted RFLP marker, which is tightly linked to nts-1 gene, identified no polymorphism among the soybean cultivars studied. The goal of the present study was to identify molecular marker(s) linked to the rj7 locus using PCR-based AFLP technology. DNA was isolated using the CTAB method. Restriction digestion, PCR preamplification, and selective amplification of restriction fragments were achieved using the AFLP**TM Analysis System I (GIBCOBRL, Life Technologies) with minor modifications. Initially, the two parents (Harosoy and NOD1-3) and two F2 bulks were used for bulked segregant analysis to identify markers linked to rj7. The segregation of these markers was then analyzed in a F2 population segregating for rj7. Using segregation data from the F2 population, the genetic distances of the markers were estimated and a genetic map of the region was generated. In addition, the inheritance of the markers was confirmed by analysis of the segregation of the markers in F4 lines derived from the F2 lines. We conclude that the AFLP analysis was a useful technique to identify markers tightly linked to the rj7 gene.