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United States Department of Agriculture

Agricultural Research Service

Title: Influence of Carbon-to-Nitrogen Ratio, Carbon Concentration, and Amino Acid Composition of Growth Media on Conidiation of Helminthosporium Solani

Authors
item Elson, Marshall - BLACKSBURG, VA
item Schisler, David
item Jackson, Mark

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: March 20, 1998
Publication Date: N/A

Technical Abstract: Silver scurf is an important storage disease of potatoes. Experiments designed to evaluate controls for this disease are limited by difficulty in producing conidia on solid-phase basal salts media. This study was conducted to determine how various carbon-to-nitrogen (C:N) ratios, carbon concentrations, and amino acids affect conidiation of H. solani. Under the conditions tested, the highest concentrations of conidia were produced with 1.25 to 2.5 g of carbon/L at a C:N ratio of 10:1. Higher C:N ratios or higher carbon concentrations reduced conidiation. Total conidia production improved with tyrosine or arginine as the sole nitrogen source. Leucine, methionine, or phenylalanine as the sole nitrogen source severely inhibited conidia production of H. solani. Use of a nitrogen source containing a mixture of amino acids resulted in a defined medium permitting conidiation of H. solani similar to or better than the level obtained with standard V8 juice medium.

Last Modified: 9/1/2014
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