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United States Department of Agriculture

Agricultural Research Service

Title: Structure of the Gene for Porcine Endometrial Folate Binding Protein

Authors
item Vallet, Jeffrey
item Smith, Timothy
item Sonstegard, Tad
item Heaton, Michael

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: June 29, 1998
Publication Date: N/A

Technical Abstract: Two different cDNAs have been isolated from porcine endometrium, corresponding to putative secreted (binding protein) and membrane (receptor) forms of this protein. In the current study, the gene for the putative secreted FBP was characterized. A porcine genomic yeast artificial chromosome (YAC) library was screened by PCR using a primer pair that does not distinguish between the two forms of FBP. A positive YAC clone was the subcloned into the SuperCOS cosmid vector, and subclones were screened by PCR using primer pairs which were specific to the 3 prime end of each cDNA. A cosmid containing the putative secreted FBP gene was obtained and sequenced. The putative secreted FBP gene spanned 6221 bp. Comparison of this sequence to the cDNA sequence identified six exons (exon 1, base 1409-1434; exon 2, 1447-1462; exon 3, 2275-2697; exon 4, 4682-4870; exon 5, 5067-5202; and exon 6, 5335-5762), demonstrating that the gene structure is ssimilar to folate receptor genes described for other species. The sequence was analyzed using Bioinformatics and Molecular Analysis Section (BIMAS) signal scan, to search for possible controlling elements within the FBP gene. Three CCAAT binding factor sites, two c-Myb sites, two hepatocyte nuclear factor 3 beta sites, and a LyF-1 (lymphocyte specific transcription factor) site were all located in the region upstream from exon 1. Other noteworthy possible controlling sites within the rest of the gene included two AP-1 sites, 13 AP-2 sites and multiple SP1 sites. These results suggest several possible regulatory factors which could be involved in controlling expression of this gene. Elucidation of the controlling factors may allow improved transport of folate to the developing conceptus.

Last Modified: 11/28/2014