|Green, M. - PLNT SCI, NDSU, FARGO, ND|
|Horsley, R. - PLNT SCI, NDSU, FARGO, ND|
|Schwarz, P. - CER SCI, NDSU, FARGO, ND|
Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: January 28, 1998
Publication Date: N/A
Technical Abstract: The enzyme alpha-amylase is an important quality characteristic in malting barley. However, the genetics of this quantitative trait are not well understood. Crosses between closely related cultivars that differ in alpha-amylase activity offer an ideal population for genetic studies of this enzyme. The objectives of this study were to i) identify molecular markers associated with alpha-amylase activity in a cross between closely related barley cultivars that differ in alpha-amylase activity, ii) determine the linkage distance between marker(s) and major gene(s), and iii) locate the molecular marker(s) to chromosome(s). A doubled-haploid population and an F3-derived population from the cross Stander (high alpha-amylase) by Robust (low alpha-amylase) were used. A RAPD marker was identified that was associated with alpha-amylase activity and explains 12.9 to 20.0% of the variation. An RFLP clone mapped to chromosome 3 was also found to be associated with alpha-amylase activity and explains 11.4 to 25.9% of the variation.