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United States Department of Agriculture

Agricultural Research Service

Title: Bioconversion of Oleic Acid to a Series of Keto-Hydroxy and Dihydroxy Acidsby Bacillus Strain Nrrl Bd-447

item Lanser, Alan
item Manthey, Linda
item Berry, Katherine - ARS/USDA/NCAUR, PEORIA,IL
item Bene, Henry - ARS/USDA/NCAUR, PEORIA,IL

Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: May 13, 1998
Publication Date: N/A

Technical Abstract: Fifty-eight strains of eight Bacillus species were selected from the ARS Culture Collection to investigate their ability to bioconvert oleic acid to unique hydroxy or keto derivatives. Addition of 0.5% oleic acid substrate to a whole-cell culture of Bacillus strain NRRL BD-447 produced mixtures of mono-, di- and tri-substituted compounds. These products appear to contain combinations of keto- and hydroxy- groups. One of the major products, 7-hydroxy-16- keto-9-octadecenoic acid, was isolated by HPLC and its structure determined by GC-MS and NMR. Isolation and identification of other major products is described.

Last Modified: 4/18/2015
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