|Jin, Wei - IOWA STATE UNIVERSITY|
|Horner, Harry - IOWA STATE UNIVERSITY|
Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 15, 1998
Publication Date: N/A
Interpretive Summary: Male-sterile, female-fertile mutants are found in many plant species. In crops such as soybean, these mutants are useful for certain plant breeding methods. If the plant breeder wishes to transfer the trait to other soybean plants, it is very time consuming. The reason is that in every other generation of transfer the sterile trait is marked or hidden by the fertile trait. By the use of biochemical markers, the fertile trait can b identified because the biochemical marker is located close to this fertile trait. This linkage that we have identified can be used in the field or the greenhouse by the plant breeder. This technique to detect genetic associations is more efficient because fewer numbers of plants need to be grown and plants can be identified for fertility/sterility before flowering. This research will facilitate soybean breeders in developing hybrid soybeans which may be more vigorous than current ones.
Technical Abstract: A newly identified genic male-sterile mutant in soybean has high seed set under natural field conditions and is potentially useful in hybrid seed production. Sterility in this mutant is caused by failure of callose dissolution at the tetrad stage, which results in microspore abortion; however, little is known about the male-sterile gene at the molecular level. The objective of this study was to identify molecular markers linked with the male-sterile gene (ms) and to place the ms gene onto the soybean molecular genetic map. An F2 population of 111 individuals was constructed from a cross between the mutant msMOS (ms ms) and the cultivar Minsoy (Ms Ms). Two hundred and seventy probes, including 19 RFLP and 51 SSRs, were evaluated. Of these, 102 RFLP probes and 31 SSR markers detected polymorphisms between the parents. The F2 population was screened for segregation of these polymorphic molecular markers. Analyses revealed that the male-sterile locus, designated ms', was located on linkage group D1b of the USDA/ARS/ISU soybean molecular genetic map. The availability of linked DNA markers will facilitate the genetic analysis of this male- sterility gene in relation to soybean breeding programs, and will be a starting pont for the isolation of the ms gene by map-based cloning.