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United States Department of Agriculture

Agricultural Research Service

Title: Construction of An Rflp Linkage Map for Cultivated Sunflower

Authors
item Jan, Chao-Chien
item Vick, Brady
item Miller, Jerry
item Kahler, A - BIOGENETIC SERVICES, INC.
item Butler, Iii, E - GAEA, INC.

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 19, 1997
Publication Date: N/A

Interpretive Summary: An RFLP linkage map of cultivated sunflower, Helianthus annuus L., was constructed based on the segregation of 269 loci detected by 228 cDNA probes. Ninety-three F2 plants of a cross between inbred lines RHA 271 and HA 234 were used as the mapping population. The linkage analyses were conducted using MAPMAKER 3.0. The genetic markers defined 20 linkage groups, covering 1129 centiMorgans of the sunflower genome. One locus was unlinked and four loci failed to be assigned onto their respective sequences. One hundred ninety-nine of the 269 loci had codominance segregation, with the rest showing dominance segregation. Among the mapped 226 probes (264 loci), 32 segregated for multiple loci, and 43 clusters of tightly linked markers with 0 cM distance among loci. This map has an average marker to marker distance of 4.6 cM, with seven markerless regions exceeding 20 cM. More work is needed to provide a better marker coverage of the entire genome, and to reduce the linkage group to 17, the 2n chromosome number of cultivated sunflower.

Technical Abstract: An RFLP linkage map of cultivated sunflower, Helianthus annuus L., was constructed based on the segregation of 269 loci detected by 228 cDNA probes. Ninety-three F2 plants of a cross between inbred lines RHA 271 and HA 234 were used as the mapping population. The linkage analyses were conducted using MAPMAKER 3.0. The genetic markers defined 20 linkage groups, covering 1129 centiMorgans of the sunflower genome. One locus was unlinked and four loci failed to be assigned onto their respective sequences. One hundred ninety-nine of the 269 loci had codominance segregation, with the rest showing dominance segregation. Among the mapped 226 probes (264 loci), 32 segregated for multiple loci, and 43 clusters of tightly linked markers with 0 cM distance among loci. This map has an average marker to marker distance of 4.6 cM, with seven markerless regions exceeding 20 cM. More work is needed to provide a better marker coverage of the entire genome, and to reduce the linkage group to 17, the 2n chromosome number of cultivated sunflower.

Last Modified: 12/18/2014
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