ARS | Publication request: DEVELOPMENT OF MICROSATELLITE MARKERS IN THE REGION OF THE PORCINE RYANODINE RECEPTOR GENE FROM A MICRODISSECTED SWINE CHROMOSOME 6 GENOMIC LIBRARY

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: February 14, 1998
Publication Date: N/A

Technical Abstract: One of the major goals of livestock genome research is to identify the quantitative trait loci (QTL) that control production traits. A critical tool for the development of genetic linkage maps has been the identification of polymorphic microsatellite (MS) markers. To facilitate the identification of MS markers in the region of the porcine skeletal muscle ryanodine receptor gene, a microdissected genomic library was generated from one third of the q arm of swine chromosome 6 proximal to the centromere. Purified DNA was digested with the restriction enzyme Sau3A I, ligated to oligonucleotide adaptors and amplified by PCR using primers complementary to the adaptor sequences. The purity of the amplified products and boundaries of the microdissected chromosomal region were verified by fluorescence in situ hybridization. A small insert genomic library of 2.3 x 106 clones was generated by screening this library with a 32P-labeled (GT)10 oligonucleotide probe. PCR primers were developed for 30 of the 49 (CA)n repeat-containing clones. These 30 unique MS markers have been tested on 20 unrelated animals from 6 different breeds and polymorphic markers (n=24) are currently being genotyped on the University of Illinois Yorkshire x Meishan swine reference family and assigned on the swine linkage map.