Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: May 13, 1998
Publication Date: N/A
Technical Abstract: The bioconversion of oleic acid, linoleic acid, and ricinoleic acid by Pseudomonas aeruginosa PR3 was investigated. The conversion of oleic acid to 7,10 dihydroxy 8(E) octadecenoic acid (DOD) was improved from 60% to greater than 80% yields by modifying the culture medium and reaction parameters. The microbial culture was maintained to retain the same level of DOD production capacity for up to six months. Strain PR3 did not bioconvert linoleic acid. However, strain PR3 converted ricinoleic acid to a novel compound, 7,10,12 trihydroxy 8(E) octadecenoic acid (TOD), with a yield of about 35%. The product was further metabolized by strain PR3. TOD was purified by thin-layer chromatography, and its structure was determined by 1H- and 13C-nuclear magnetic resonance and mass spectroscopy. This is the first report on the production of 7,10,12 trihydroxy unsaturated fatty acid by microbial transformation.